ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 40-45 kDa band in human cerebellum lysates after 0.2 μg/mL antibody staining. Immunohistochemistry: In paraffin embedded human kkeletal muscle shows striated staining in alternating fibres. Recommended concentration, 5-10 μg/mL.
PCSK6 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PCSK6 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.