ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 90 kDa band observed in human cerebellum lysates (calculated MW of 99.2 kDa according to NP_001070711.1). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 5-10 μg/mL.
GRIA4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GRIA4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.