ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 48 kDa band observed in human brain (cerebellum and cerebral cortex) lysates (calculated MW of 47.8 kDa according to NP_071429.1). Recommended concentration: 0.03-0.1 μg/mL.
NPFFR1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NPFFR1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.