ELISA: Antibody detection limit dilution 1:64000. Western Blot: In transfected HEK293 transiently expressing NOVA1 a band of approximately 52 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded human cerebral cortex shows staining of selected nuclei. Recommended concentration, 2-4 μg/mL.
NOVA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NOVA1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.