ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 45 kDa band observed in human skeletal muscle lysates (calculated MW of 43.6 kDa according to NP_034732.1). Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas shows variable staining across the islet of Langerhans. Recommended concentration, 3-5 μg/mL.
KCNJ11 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
KCNJ11 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.