ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 28 kDa band observed in human breast tumor lysates (calculated MW of 31.7 kDa according to NP_001013416.1 and 32.2 kDa according to NP_000589.2). Recommended concentration: 0.0
IGFBP3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
IGFBP3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.