ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Approximately 50 kDa band observed in human lymph node lysates (calculated MW of 50.9 kDa according to NP_004742.1). In transfected HEK293 transiently expressing GCNT3 a band of approximately 55 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL.
GCNT3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GCNT3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.