ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 60 kDa band observed in human colon and small intestine (Ileum) lysates (calculated MW of 59.9 kDa according to NP_036325.2). Recommended concentration: 0.3-1 μg/mL.
Frizzled 4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Frizzled 4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.