ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 50 kDa band observed in human cerebellum and breast lysates (calculated MW of 51.3 kDa according to NP_NP_001429.2). In transfected HEK293 transiently expressing ESRRG a band of approximately 52 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 μg/mL.
ESRRG antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ESRRG antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.