ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 170 kDa band observed in Jurkat lysate (calculated MW of 183 kDa according to NP_001370.1). Recommended concentration: 0.5-1.5 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows nuclear staining in PCT. Recommended concentration, 3-6 μg/mL.
DNMT1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DNMT1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.