ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 65-70 kDa band in human heart and thyroid lysates after 0.05 μg/mL antibody staining.5 kDa according to NP_NP_000784.2 and of 34.6 kDa according to NP_001007024.1. The 65-70 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: Cryo-sectioned mouse hippocampus shows staining in the granular layer of the dentate gyrus. Recommended concentration, 2-3 μg/mL. Data obtained from anonymous customer.
DIO2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DIO2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse