ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 65-70 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 65.3 kDa according to human NP_444511.1 and of 68.6 kDa according to mouse NP_291083.1).
DACH2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
DACH2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.