ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 55-60 kDa band observed in human liver and Ileum lysates (calculated MW of 62.6 kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approximately 65 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/mL. Immunohistochemistry: In paraffin embedded human lung shows staining of lymphocytes in the vasculature. Recommended concentration, 2-3 μg/mL.
CES1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CES1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.