ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 45 kDa band observed in human liver, mouse liver and rat liver lysates (calculated MW of 44.9 kDa according to human NP_001704.1, 45.0 kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approximately 49 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows strong cytoplasm staining in PCT cells. Recommended concentration, 2-4 μg/mL.
BHMT antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
BHMT antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse, Rat