ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 26 kDa band observed in human heart and muscle lysates (calculated MW of 25.7 kDa according to NP_006780.1). Recommended concentration: 1-3 μg/mL. an additional band of 90 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.
APOBEC2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
APOBEC2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.