ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 18 kDa band in human brain lysates after 1 μg/mL antibody staining.3 kDa according to NP_848018.1 and 35.8 kDa according to NP_777568.1. The 18 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human pancreas shows strong staining in cytoplasm in select acinar cells. Recommended concentration, 5-10 μg/mL.
Ymer antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Ymer antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.