ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 38 kDa band observed in human brain lysates (calculated MW of 39.6 kDa according to NP_110380.1). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human breast shows strong fibrous staining in myoepithelial cells. Recommended concentration, 3-5 μg/mL.
WNT3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
WNT3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse