ELISA: Antibody detection limit dilution 1:64,000. Approximately 60 kDa band observed in human lung, spleen and thymus lysates (calculated MW of 30.9 kDa according to NP_078902.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Simon et al, tumor Res. 2006 Feb 1;66 (3) :1570-5.; PMID: 16452214). In transfected HEK293 transiently expressing B7-H4 the band of approximately 60 kDa is also observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL.
VTCN1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
VTCN1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.