ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 90 kDa band observed in human brain and testis lysates (predicted MW of 91 kDa according to NP_060676.2). Recommended for use at 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis.
VPS35 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
VPS35 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.