ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 75 kDa band in human duodenum, colon and placenta lysates after 0.3 μg/mL antibody staining.3. The 75 kDa band was successfully blocked by incubation with the immunizing peptide.
VPS11 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
VPS11 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.