ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 56 kDa band observed in human lung lysates (calculated MW of 52.9 kDa according to NP_000574.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (DiMartino and Kew, J Immunol. 1999 Aug 15;163 (4) :2135-42; PMID: 10438954). Recommended concentration: 0.1-0.3 μg/mL.
Vitamin D-binding Protein antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Vitamin D-binding Protein antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.