ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 120 kDa band observed in human testis lysates and in Jurkat lysate (calculated MW of 110 kDa according to NP_004642.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ideguchi et al, Biochem J. 2002 Oct 1;367 (Pt 1) :87-95.; PMID: 12084015). Recommended concentration: 1-3 μg/mL.
USP11 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
USP11 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.