ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 105 kDa band observed in human T-lymphocyte and HeLa lysates (calculated MW of 123 kDa according to NP_954712.1). Recommended concentration: 1-3 μg/mL. Immunohistochemisty: In paraffin embedded human thymus shows cytoplasm staining in select areas. Recommended concentration, 2.5 μg/mL.
UNC13D antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
UNC13D antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse