ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 35 kDa band observed in human adipose lysates (calculated MW of 33.0 kDa according to NP_068605.1). Recommended concentration: 0.1-1 μg/mL.
UCP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
UCP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.