ELISA: Antibody detection limit dilution 1:128,000. Approximately 35 kDa band observed in human brain lysates (calculated MW of 18.8 kDa according to NP_000705.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Delavoie et al, Biochemistry. 2003 Apr 22;42 (15) :4506-19.; PMID: 12693947). Recommended concentration: 0.1-0.3 μg/mL. Approximately 17 kDa band observed in human breast carcinoma lysates (calculated MW of 18.8 kDa according to NP_000705.2). Recommended concentration: 0.1-0.3 μg/mL.
TSPO antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TSPO antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.