ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 150 kDa band observed in human brain lysates (calculated MW of 130 kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22;9 (12) :1721-7; PMID: 10915759). In transfected HEK293 transiently expressing human TSC1 a band of approximately 120 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/mL.
TSC1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TSC1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.