ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 150 kDa band observed in human cerebellum lysates (calculated MW of 213 kDa according to NP_060142.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Clark et al PLoS ONE. 2008 Mar 26;3 (3) :e1876.; PMID: 18365021). Recommended concentration: 0.3- 1 μg/mL. Immunohistochemistry: In paraffin embedded mouse brain shows neuronal staining in the hippocampus. Recommended concentration, 4-6 μg/mL.
TRPM7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TRPM7 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Mouse, Rat