ELISA: Antibody detection limit dilution 1:2000. Approximately50 kDa band observed in human testis lysates (calculated MW of 53.3 kDa according to NP_005870.2). Recommended concentration: 2.0 ug/ml. an additonal band of unkown identity was also concistently observeved at 38 kDa . This band was successfully blocked by incubation with the immunizing peptide.
TRIP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TRIP antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.