ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 50-55 kDa band observed in lysates of monocytic leukemia cell line U937 (calculated MW of 46.2 kDa according to NP_005649.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pryhuber et al, Am J Respir Cell Mol Biol. 2000 Feb;22 (2) :150-6. PMID: 10657935). Recommended concentration: 0.3-1.0 μg/mL.
TRAF1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TRAF1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.