ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 26-28 kDa band observed in human lymph node and human kidney lysates (calculated MW of 24.3 kDa according to NP_001020423.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tiacci et al, Blood. 2005 Apr 1;105 (7) :2812-20.; PMID: 15576473). Recommended concentration: 1-3 μg/mL.
TPD52 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TPD52 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.