ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 24 kDa band observed in lysates of human myeloid erythroblast call line K562 (calculated MW of 23.9 kDa according to NP_001034750.1 and 24.8 kDa according to NP_683708.1). Recommended concentration: 0.1-0.3 μg/mL.
TIRAP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TIRAP antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.