ELISA: Antibody detection limit dilution 1:16,000. Western Blot: experiments gave bands at approximately 40 kDa in human heart lysate after 0.5 μg/mL antibody staining. This band corresponds to earlier findings in literature with different antibodies (PMID: 15044455). This protein has a calculated MW of 50.5 kDa according to NP_001001563. Recommended concentration: 0.5-3 μg/mL.
TIM-50 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TIM-50 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.