ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 120 kDa band in human kidney and heart lysates at 1 μg/mL, however prominent bands were also seen at 25 kDa and 45 kDa . Immunohistochemistry: In paraffin embedded human breast carcinoma shows nuclear staining in the distorted lobules. Recommended concentration, 4-6μg/ml
TIF1 alpha antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TIF1 alpH a antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.