ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 40 kDa band observed in Jurkat lysate (calculated MW of 41.8 kDa according to NP_071320.1). Recommended concentration: 0.1-0.3 μg/mL. an additional band at 80 kDa and a very strong 26 kDa is also observed. These bands were successfully blocked by incubation with the immunizing peptide.
TIA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TIA1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.