ELISA: Antibody detection limit dilution 1:2000. Western Blot: No signal obtained yet but low background observed in 293 and A549 lysates at up to 2 μg/mL (please note that our SDS PAGE/Western Blotting protocol is not optimized for detection of proteins smaller than 15 kDa ).
Thymosin-like 8 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Thymosin-like 8 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.