ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave one band at approximately 37 kDa and much stronger bands at approximately 70+28 kDa in mouse testis lysates after 0.5 μg/mL antibody staining.1 kDa according to mouse NP_112475.1. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Tcfec antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Tcfec antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.