ELISA: Antibody detection limit dilution 1:8000. Western Blot: In transfected HEK293 transiently expressing full-length human MAP3K7 (myc and DYKDDDDK tagged), a band of approximately 75 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 μg/mL.
TAK1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
TAK1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.