ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 70 kDa band observed in human duodenum and human Ileum lysates (calculated MW of 60.2 kDa according to NP_061841.1). Recommended concentration: 0.1-0.3 μg/mL. an additional band of unknown identity was also consistently observed at 110 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Syntrophin gamma 2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SyntropH in gamma 2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.