ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 80 kDa band observed in rat brain lysates (calculated MW of 82.6 kDa according to NP_476558.1 (rat). Recommended concentration: 0.3-1 μg/mL. an additonal band of unknown identity was also consistently observed at 28 kDa . This band was successfully blocked by incubation with the immunizing peptide.
SV2A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SV2A antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.