ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 150 kDa band observed in HeLa lysate (calculated MW of 120 kDa according to NP_009123.1). Recommended concentration: 0.1-0.3 μg/mL. an additional band of unknown identity was also consistently observed at 30 kDa . This band was successfully blocked by incubation with the immunizing peptide.
SUPT16H antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SUPT16H antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.