ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 30 kDa band in human adipose, brain and liver lysates after 0.3 μg/mL antibody staining.5 kDa according to NP_005054.3. The 30 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human liver shows textured cytoplam staining in hepatocytes. Recommended concentration, 5-10 μg/mL.
Stearoyl-CoA desaturase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Stearoyl-CoA desaturase antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.