ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 70 kDa band observed in human spleen lysates (calculated MW of 58.2 kDa according to NP_005834.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pandey et al J Biol Chem. 2000 Dec 8;275 (49) :38633-9; PMID: 10993906). Recommended concentration: 1-3 μg/mL.
STAM2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
STAM2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.