ELISA: Antibody detection limit dilution 1:16000. Western Blot: this antibody has been successfully used in ERMS cells (PMID: 20068162). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: this antibody has been successfully used by anonymous customers in paraffin embedded mouse brain, showing nuclear staining. Recommended concentration: 1-3 μg/mL.
Sprouty antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Sprouty antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse