ELISA: Antibody detection limit dilution 1:32,000. Western Blot: experiments gave bands at approximately 50 kDa and 29 kDa in human cerebral cortex lysates and in mouse and rat brain lysates after 0.5 μg/mL antibody staining. These bands correspond to earlier findings in literature with different antibodies (Yamaguchi et al, J Biol Chem. 2002 Mar 1;277 (9) :6806-12; PMID: 11741986). This protein has a calculated MW of 49.6 kDa according to NP_110397.1. Recommended concentration: 0.5-1.5 μg/mL.
Spinesin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Spinesin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse, Rat