ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Not yet tested - our routinely used Western Blotting protocol does not allow detection of proteins as large as the calculated size of approximately 402 kDa according to NP_055816.2. Therefore we cannot recommend an optimal concentration and the product is investigative grade.
SPEN antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPEN antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.