ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 20 kDa band in rat brain and rat spinal cord lysates after 1 μg/mL antibody staining.6 kDa according to mouse NP_035571.1 and of 44.1 kDa according to human NP_071899.1. The 20 kDa band was successfully blocked by incubation with the immunizing peptide.
SOX17 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SOX17 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.