ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 26 kDa band in lysates of cell line KELLY after 0.3 μg/mL antibody staining. The 26 kDa band was successfully blocked by incubation with the immunizing peptide.
SOX1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SOX1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.