ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Experiments gave bands at approximately 48 kDa and 24 kDa in human brain lysate after 0.3 μg/mL antibody staining.2 kDa according to NP_054730 and NP_659198. Both detected bands were successfully blocked by incubation with the immunizing peptide. Approximately 65 kDa band observed in Molt4 lysate (calculated MW of 61.2 kDa according to NP_054730.1). Recommended concentration: 0.3-1 μg/mL.
SOCS5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SOCS5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.