ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 48 kDa band observed in lysates of human kidney and cell lines Jurkat and A549 lysates (calculated MW of 46.8 kDa according to NP_055241.1). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human breast shows cytoplasm staining of ductal epithelial cells. Cytoplasm staining is also observed in PCT of human kidney and in trophoblasts of human placenta . Recommended concentration, 3-6 μg/mL.
SNX5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SNX5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.