ELISA: Antibody detection limit dilution 1:1,000. Approximately 60 kDa band observed in human bone marrow lysates (calculated MW of 86.4 kDa according to NP_005622.1). The observed molecular weight corresponds to findings with 46-388, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.1-0.3 μg/mL.
SMO antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SMO antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.